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How to measure emulsification index (E24) 1 Citation In biotechnology field, this method is used to measure the emulsification ability of biosurfactant. Emulsification index (E24) is used to characterize the biosurfactant in emulsifying, generally, the hydrophobic phase in hydrophilic phase. Following s are the emulsification index procedure based on the published journal titled ?The production of rhamnolipid by a Pseudomonas aeruginosa strain isolated from a southern coastal zone in Brazil? by L.M. Prieto, et al.: adding 2 ml of sample to, technorati.com
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Measurement of Biosurfactant Activity 1 Citation Biosurfactant activity was measured by an oil displacement test. This method is so sensitive that only a small amount of sample is required to measure the surfactant activity. Here is the method: A sample is solubilized or suspended in 10 micro liters of 10% acetonitrile or 0.1 M Tris-Cl (pH 8) buffer. After solubilized or suspended , a sample was put on the center of an oil membrane which was formed on the surface of water in a large size petri dish (15 cm diameter). The size of the resu, technorati.com
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Surfactant Properties: How to Quantitatively Measure 1 Citation In order to measure the properties of surfactant, we can use the surface tension, emulsification activity, and hemolytic activity as the parameters. Here are the methods: Surface tension analysis was carried out using the method of Makkar et al. Aqueous solutions of MC and PPGF were prepared at concentrations of 0.1, 0.5, 1, 2.5 and 5 mg/ml. Triton X-100 well known chemically synthesized surfactant was employed as a control. The surface tension of each sample was measured five times us, technorati.com
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